Whiteboard Chemistry with Joe White

NMR Spectroscopy

How 13C and 1H NMR reveal a molecule’s carbon skeleton and hydrogen environments — chemical shift, integration and the n+1 splitting rule — and how to work backwards from a spectrum to a structure.

AQA 7404/7405 Paper 2 A-level only
CH₃CH₂OH 0 δ

Why NMR? The structure-solving toolkit

No single instrument tells you a molecule’s structure. In practice chemists run a compound through a set of techniques and let each one contribute what it does best. You met the first two at AS in Organic analysis (3.3.6): mass spectrometry pins down the Mr — and, at high resolution, the molecular formula — while infrared spectroscopy identifies the functional groups from their absorptions.

That is usually where the trail goes cold: a molecular formula and a functional group still fit several isomers. Nuclear magnetic resonance (NMR) is the technique that finishes the job. It reports on the position of every 13C and 1H atom in the molecule — how many distinct environments there are, how the hydrogens are shared between them, and even what sits on the neighbouring carbon. Where infrared names a functional group and mass spectrometry weighs the molecule, NMR draws you the map.

MASS SPEC INFRARED ¹³C NMR M⁺ = 60 m/z broad O–H wavenumber 3 environments δ / ppm Mᵣ = 60 → C₃H₈O O–H → an alcohol 3 C environments CH₃CH₂CH₂OH propan-1-ol — not propan-2-ol (2 environments)
One compound, three instruments: mass spec weighs it, infrared names the functional group, NMR settles which isomer it is.
The big idea

NMR looks at atoms with nuclear spin — 13C and 1H — and reports on their environment. 13C NMR counts the carbon environments; 1H NMR is richer, telling you how many hydrogen environments there are, how many H are in each, and what sits on the neighbouring carbon. The exam skill is not theory — it is reading a spectrum backwards to a structure.

TMS, solvents & the δ scale

Every peak position is measured relative to a standard, tetramethylsilane (TMS, Si(CH3)4), which is assigned δ = 0. The horizontal axis is the chemical shift δ in parts per million (ppm); the further a signal is from electronegative atoms and double bonds, the closer it sits to TMS.

Why TMS is the standard
  • Its 12 hydrogens are all equivalent, so it gives a single sharp signal.
  • It is non-toxic and inert (won’t react with the sample).
  • It is volatile (bp 26 °C), so it is easily removed afterwards.
  • Its signal is upfield of almost all others (to the right, at δ 0), so it doesn’t overlap the sample’s peaks.

The sample is dissolved in a solvent with no 1H atoms — otherwise the solvent’s own protons would appear. Common choices are CCl4 (no hydrogen at all) or deuterated solvents such as CDCl3, where the hydrogen is replaced by deuterium (2H) — so there is no 1H present to give a signal in the 1H spectrum.

13C vs 1H at a glance

13C NMR is the simpler spectrum: one peak per carbon environment, positions from the Data Booklet — no splitting or integration to consider. 1H NMR adds two more layers of information: integration (how many H per environment) and splitting (what’s on the neighbouring carbon).

🧪 Exam-style questions
Q1 [1 mark]

Why does tetramethylsilane (TMS) give only one peak in a 1H NMR spectrum?

  1. It contains no hydrogen atoms.
  2. All twelve of its hydrogen atoms are in the same environment.
  3. It has a low boiling point.
  4. It is chemically inert.
The four methyl groups are equivalent, so all twelve H atoms share one environment → one signal. (The low bp and inertness are reasons TMS is convenient, not why it gives one peak.)
Q2 [6 marks]

A compound is mixed with Si(CH3)4 and either CCl4 or CDCl3 before recording its 1H NMR spectrum. State why each substance is used and explain how its properties make it suitable.

Show answer

Si(CH3)4 (TMS) is the reference / standard, giving the peak at δ = 0. It has 12 equivalent H (one signal), is inert (won’t react with the sample), and is volatile / has a low bp (easily removed). Its signal is away from most others. up to 3 marks

CCl4 / CDCl3 are solvents. Both contain no 1H, so give no signals of their own (in CDCl3 the hydrogen is replaced by deuterium, 2H). CCl4 is non-polar (dissolves non-polar samples); CDCl3 is polar (dissolves polar samples). up to 3 marks

Source: AQA A-Level Chemistry past papers.

13C NMR — counting carbon environments

A 13C spectrum has one peak for each carbon environment. Carbons are in the same environment if they are equivalent by symmetry. So the first job is always to count how many different carbons the molecule has — that is the number of peaks.

The position of each peak (its chemical shift) tells you the type of carbon, read from Table C of the Data Booklet. As in Table B, each row describes one carbon — the red C below (bold in the booklet):

Table C — 13C NMR chemical shift data, as in the Data Booklet
Type of carbonδ / ppm
C–C (alkyl)5–40
R–C–Cl  or  R–C–Br10–70
R–C–C=O  (C next to a carbonyl)20–50
R–C–N  (amines)25–60
C–O  (alcohols, ethers, esters)50–90
C=C  (alkene)90–150
R–C≡N  (nitrile)110–125
Aromatic C110–160
R–C=O  (esters or acids)160–185
R–C=O  (aldehydes or ketones)190–220
CH₃ C O O CH₂ CH₃ a b c d 4 carbon environments → 4 peaks b c a d 200150100500 δ / ppm C=O ester 160–185 C–O 50–90
Ethyl ethanoate: four distinct carbons, four peaks — the two CH3 groups are in different environments, so they do not share a peak. Symmetry works the other way: equivalent carbons share one peak, so a symmetrical molecule gives fewer.
🧪 Exam-style questions
Q3 [1 mark]

How many peaks are in the 13C NMR spectrum of propan-2-one (propanone), CH3COCH3?

  1. 1
  2. 2
  3. 3
  4. 4
The two CH3 groups are equivalent (one environment) and the C=O is a second — so 2 peaks, not 3. Symmetry reduces the count.

Source: AQA A-Level Chemistry past papers.

1H NMR — shift, integration & splitting

A 1H spectrum carries three pieces of information at once: where each signal sits (chemical shift), how big it is (integration), and how many lines it is split into (spin–spin splitting). Read them in that order for every signal — each layer answers a different question about the molecule.

1. Chemical shift — what kind of environment?

One signal per hydrogen environment, and the signal’s chemical shift δ tells you the type of environment, read from Table B of the Data Booklet. The pattern behind the table: the nearer the hydrogen is to an electronegative atom (O, Cl) or to a C=O, the more the electron density around it is pulled away and the larger its shift — the peak moves left, away from TMS.

Read each row carefully: it describes one hydrogen only — shown in red below (the Data Booklet prints it in bold). Everything else in the fragment is just scenery telling you where that H sits.

Table B — 1H NMR chemical shift data, as in the Data Booklet (the red H is the one giving the peak)
Type of protonδ / ppm
ROH0.5–5.0
RCH30.7–1.2
RNH21.0–4.5
R2CH21.2–1.4
R3CH1.4–1.6
R–CO–C–H  (H on a C next to a C=O)2.1–2.6
R–O–C–H  (H on a C bonded to O)3.1–3.9
RCH2Cl  or  RCH2Br3.1–4.2
R–CO–O–C–H  (H on a C bonded to an ester O)3.7–4.1
R2C=CH–  (alkene)4.5–6.0
R–CHO  (aldehyde)9.0–10.0
R–COOH  (carboxylic acid)10.0–12.0
← larger δ : H pulled by O, Cl or C=O TMS at δ 0 H–C–C=O RCH₃ · R₂CH₂ · R₃CH RCH₂–X C=C–H R–CHO R–COOH R–O–C–H ester R–CO–O–C–H (3.7–4.1) RO–H: anywhere 0.5–5.0 R–NH₂: anywhere 1.0–4.5 121086420 δ / ppm
Every Table B row on one ruler. Note the crowded patch around δ 3–4: the halogen row (3.1–4.2), the C–O row (3.1–3.9) and the ester row (3.7–4.1) all overlap — when more than one row fits a peak, let the molecular formula decide.

2. Integration — how many H?

The area under each signal is proportional to the number of H atoms in that environment. On AQA papers the integration is usually given as a number printed above each peak (sometimes with a small integral step drawn over the peak instead). Convert the numbers to the simplest whole-number ratio: a 1.2 : 1.2 : 1.8 integration is a 2 : 2 : 3 ratio of hydrogens.

One trap: the ratio is not automatically the number of H. Check it against the molecular formula — if C4H8O shows a 3 : 2 : 3 ratio, the total (8) matches and the counts really are 3H, 2H, 3H; but a single-peak spectrum of C3H6O with “ratio 1” is six hydrogens, not one.

BUTANONE · CH₃COCH₂CH₃ relative area above each peak 2 3 3 43210 δ / ppm
Butanone: the relative areas printed over the peaks read 2 : 3 : 3 — so the peaks are 2H, 3H and 3H (total 8 H, matching C4H8O).

3. Splitting — the n+1 rule

Each signal is split by the hydrogens on the neighbouring carbon (spin–spin coupling). The number of lines is one more than the number of adjacent, non-equivalent H:

The n+1 rule (aliphatic; limited to these four)
SignalLinesH on the adjacent carbon
singlet10
doublet21
triplet32
quartet43
CH₃–CO– CH₃–CH< CH₃–CH₂ –CH₂–CH₃ 0 H next door 1 H next door 2 H next door 3 H next door 1 1 : 1 1 : 2 : 1 1 : 3 : 3 : 1 singlet · 0+1 doublet · 1+1 triplet · 2+1 quartet · 3+1
All four patterns: the red H give the signal, the blue H next door do the splitting — n neighbours give n+1 lines. Read it backwards in the exam: count lines, subtract one, and you know the H count next door.
–OH and –NH don’t play the splitting game

The H of an –OH (alcohol or acid) or –NH usually does not couple — it appears as a singlet and does not split the signal on the next carbon. A lone 1H singlet is very often an –OH or –COOH.

Patterns worth memorising
  • Ethyl group CH3CH2– → a triplet and a quartet, ratio 3 : 2.
  • –CH2–CH2two triplets, 2 : 2.
  • CH3–CH< → a doublet and a quartet, 3 : 1 (isopropyl gives a doublet, 6H).
  • CH3 next to a carbon with no H → a singlet, 3H.
ETHANOL · CH₃CH₂OH quartet — 3 H next door singlet — OH doesn’t couple triplet — 2 H next door 2H 1H 3H TMS 543210 δ / ppm δ 3.65 · H–C–O δ 1.15 · alkyl
All three layers on one spectrum: shift places each environment, integration counts its hydrogens, splitting reads the neighbours. Ethanol’s ethyl group gives the classic quartet + triplet (2:3); the OH is a non-coupling singlet.
🧪 Exam-style questions
Q4 [3 marks]

For butanone, CH3COCH2CH3, deduce the splitting pattern of each of the three 1H signals.

Show answer

CH3CO (next to C=O, no H on the adjacent carbon) → singlet (3H). 1 mark

CH2 (adjacent CH3 has 3 H) → quartet (2H). 1 mark

CH3CH2 (adjacent CH2 has 2 H) → triplet (3H). 1 mark

Source: AQA A-Level Chemistry past papers.

Working back to a structure

This is the exam skill. You are given a spectrum (and usually the molecular formula) and must build the structure. Take one peak at a time and squeeze out all three pieces of information — the fragments then click together:

The method, per peak
  1. Integration → how many H are in this environment.
  2. Splitting → how many H on the neighbour (lines − 1, by the n+1 rule).
  3. Shift → the environment (Table B) — is it near O, Cl, C=O, a ring?

Write each peak up as one line, then join the fragments. Here it is done in full for a compound C3H7ClO whose spectrum shows three peaks:

One worked spectrum, as exam-answer lines
UNKNOWN · C₃H₇ClO relative area above each peak TMS 2 2 3 543210 δ / ppm
  • δ 3.35, 3H, singlet → CH3 next to O with no H on the next atom — fragment CH3–O–
  • δ 3.65, 2H, triplet → O–CH2 next to a CH2 — fragment –O–CH2–CH2
  • δ 3.95, 2H, triplet → Cl–CH2 next to a CH2 — fragment –CH2–Cl

Join them: CH3–O–CH2–CH2–Cl.

Practise — the NMR analyser

This is how the method becomes automatic. Read a spectrum works exactly like the exam: take each peak in turn, fix its integration, splitting and Data-Booklet environment, collect the exam-answer lines, then sketch the structure on paper before revealing it. Predict a spectrum runs the drill in reverse — you fill in the analysis for a given molecule and the spectrum builds below as you go. The 13C drill does the lighter carbon version: count the environments, place the peaks.

The marks that get dropped
  • Counting environments wrong — equivalent atoms share one peak (symmetry lowers the count in both 13C and 1H).
  • Reading splitting the wrong way: lines = neighbours + 1, so a triplet means 2 H next door, not 3.
  • Splitting an –OH or –NH, or letting it split its neighbour — it usually doesn’t couple.
  • Ignoring the molecular formula — the integration H-total must add up to it.

Examiner reports note that the strongest answers state the deduction, not just the label — “triplet, so 2 H on the adjacent carbon” and “δ 3.95, so next to an electronegative atom” — before drawing the structure. Show the reasoning for each peak.

🎯 Model answer — the C3H7ClO structure deduction

C3H7ClO gives three 1H peaks: δ 3.95 (2H, triplet), δ 3.65 (2H, triplet), δ 3.35 (3H, singlet). Deduce the structure, explaining each peak. Award yourself a mark for each:

  • δ 3.95, triplet, 2H → Cl–CH2 next to a CH2.
  • δ 3.65, triplet, 2H → O–CH2 next to a CH2.
  • δ 3.35, singlet, 3H → CH3–O with no H on the adjacent atom.
  • Structure: CH3OCH2CH2Cl.
3.3.15 NMR spectroscopy — Quick-reference summary
  • Techniques combine: mass spectrometry fixes Mr and the molecular formula, infrared names the functional groups, and NMR maps the 13C and 1H environments — together they confirm a structure.
  • TMS (δ = 0): one signal, inert, volatile, upfield — the reference. Solvents CCl4 / CDCl3 have no 1H, so give no signal.
  • 13C NMR: number of peaks = number of carbon environments; positions from Data Booklet Table C. Simpler — no splitting or integration.
  • 1H NMR: number of signals = H environments; integration = ratio of H atoms; chemical shift from Table B.
  • n+1 rule: a signal is split into (number of H on the adjacent carbon) + 1 lines — singlet, doublet, triplet, quartet. –OH and –NH usually don’t couple (appear as singlets).
  • Solving a spectrum: for each peak read integration → H count, splitting → neighbours, shift → environment; assemble the fragments into the structure.

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